Item#31066 AccuGreen™ High Sensitivity dsDNA Quantitation Kit – 500 assays

 DESCRIPTION

The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher. Unlike absorbance-based measurements, AccuGreen™ Dye is highly selective for double-stranded DNA over single-stranded DNA or RNA.

The linear range of the AccuGreen™ assay is 0.1 to 100 ng of DNA per tube. DNA samples with concentrations between 5 pg/uL and 100 ng/uL may be quantified using sample volumes between 1 and 20 uL (for example, 1 uL of 100 ng/uL is 100 ng total, and 20 uL of 5 pg/uL is 0.1 ng total, which will both fall within the linear range of the assay). If you use the most common sample volume of 10 uL, the starting sample concentration should be 10 pg/uL to 10 ng/uL.

AccuGreen™ High Sensitivity Quantitation Solution also is available separately (catalog no. 31068) for customers who wish to use their own DNA standards.

For a broad range assay to quantify DNA in the range of 0.2-100 ng/uL on the Qubit® fluorometer, see the AccuGreen™ Broad Range dsDNA Quantitation Kit (catalog no. 31069).

For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear® or AccuBlue® NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range. See the table below for details on our full line of dsDNA quantitation kits.

Features

  • Optimized for use with the Qubit® Fluorometer
  • Uses the pre-programmed Qubit® dsDNA High Sensitivity assay setting
  • Linear range of assay: 0.1-100 ng dsDNA
  • Sample concentration range: 5 pg/uL to 100 ng/uL
  • Green fluorescence (Ex/Em 502/523 nm)

Kit components

  • AccuGreen™ Dye, 200X
  • DNA Quantitation Buffer, 20X Concentrate*
  • AccuGreen™ Standard 1 (0 ng/uL)
  • AccuGreen™ Standard 2 (10 ng/uL)

*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.

A genomic DNA sample quantified using the Qubit® HS kit and the AccuGreen™ HS kit. A nearly identical result was obtained with each kit.
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 Overview

The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.01-10 ng/uL.