Item#31066 AccuGreen™ High Sensitivity dsDNA Quantitation Kit – 500 assays
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher. Unlike absorbance-based measurements, AccuGreen™ Dye is highly selective for double-stranded DNA over single-stranded DNA or RNA.
The linear range of the AccuGreen™ assay is 0.1 to 100 ng of DNA per tube. DNA samples with concentrations between 5 pg/uL and 100 ng/uL may be quantified using sample volumes between 1 and 20 uL (for example, 1 uL of 100 ng/uL is 100 ng total, and 20 uL of 5 pg/uL is 0.1 ng total, which will both fall within the linear range of the assay). If you use the most common sample volume of 10 uL, the starting sample concentration should be 10 pg/uL to 10 ng/uL.
AccuGreen™ High Sensitivity Quantitation Solution also is available separately (catalog no. 31068) for customers who wish to use their own DNA standards.
For a broad range assay to quantify DNA in the range of 0.2-100 ng/uL on the Qubit® fluorometer, see the AccuGreen™ Broad Range dsDNA Quantitation Kit (catalog no. 31069).
For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear® or AccuBlue® NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range. See the table below for details on our full line of dsDNA quantitation kits.
Optimized for use with the Qubit® Fluorometer
Uses the pre-programmed Qubit® dsDNA High Sensitivity assay setting
Linear range of assay: 0.1-100 ng dsDNA
Sample concentration range: 5 pg/uL to 100 ng/uL
Green fluorescence (Ex/Em 502/523 nm)
AccuGreen™ Dye, 200X
DNA Quantitation Buffer, 20X Concentrate*
AccuGreen™ Standard 1 (0 ng/uL)
AccuGreen™ Standard 2 (10 ng/uL)
*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.01-10 ng/uL.
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